Click image to enlarge
The Apoptosis Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of total p53, phospho-p53 (Ser15), PARP (cleaved), and Caspase-3 (cleaved) in human whole cell lysates. Caspase-3 is initially synthesized as a precursor protein but is activated by proteolytic cleavage of the N-terminus to produce the p20 and p17 cleavage (ASP175) products measured in this kit. PARP becomes inactive following cleavage (ASP214), however, the small cleavage product does retain its DNA-binding capabilities, blocking access to damaged DNA and inhibiting further DNA repair.
Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.
-
-
Specifications
-
Kit Contents
-
Documentation
-
References
|
Application(s)
|
|
Phosphoproteins
,
Intracellular Signaling
|
|
|
Analyte(s)
|
|
Caspase-3, p53, PARP
|
|
|
Species
|
|
Human
|
|
|
Instrument(s)
|
|
SECTOR Imager 2400
,
MESO SECTOR S 600MM
,
SECTOR Imager 6000
,
MESO QuickPlex SQ 120MM
,
MESO QuickPlex SQ 120
,
MESO SECTOR S 600
|
|
|
Plate Type
|
|
96-well
|
|
|
Capture Antibody
|
|
Cleaved PARP: Rabbit Polyclonal
Cleaved Caspase-3: Rabbit Polyclonal
Phospho-p53: Mouse Monoclonal
p53: Mouse Monoclonal
|
|
|
Detection Antibody
|
|
Cleaved PARP: Rabbit Polyclonal
Cleaved Caspase-3: Goat Polyclonal
Phospho-p53: Goat Polyclonal
p53: Goat Polyclonal
|
|
|
Usage Statement
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
|
|
Kit Model
|
|
Whole Cell Lysate
|
|
|
Storage Statement(s)
|
|
Please refer to the product insert for the storage conditions of individual kit components.
|
|
|
Storage Condition
|
|
Multi-Component
|
|
|
Schedule B Code
|
|
3002.15.0100
|
|
|
Product Description
|
Storage
|
Quantity per Kit
|
|
Phosphatase Inhibitor I 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
Phosphatase Inhibitor II 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
Protease Inhibitor Solution 0.5mL
|
2-8 °C
|
1 each
(1 each)
|
|
|
|
SULFO-TAG Anti-PARP Antibody
|
2-8 °C
|
1 vial
(375 μL)
|
|
|
|
SULFO-TAG Anti-Caspase-3 Antibody
|
2-8 °C
|
1 vial
(375 μL)
|
|
|
|
SULFO-TAG Anti-p53 (1b, apoptosis) Antibody
|
2-8 °C
|
1 vial
(375 μL)
|
|
|
|
Apoptosis Panel Plate
|
2-8 °C
|
5 each
(1 each)
|
|
|
|
|
2-8 °C
|
1 bottle
(50 mL)
|
|
|
|
|
2-8 °C
|
1 bottle
(200 mL)
|
|
|
|
|
RT
|
1 bottle
(50 mL)
|
|
|
|
|
RT
|
1 bottle
(15 g)
|
|
|
|
|
RT
|
1 bottle
(1 g)
|
|
|
|
|
≤-10 °C
|
1 vial
(0.2 mL)
|
|
|
|
Product Inserts
|
|
|
|
Datasheets
|
|
|
|
Certificate of analysis
|
|
|
|
SDS
|
|
|
|
Title
|
Journal
|
Year
|
|
Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
|
|
|
|
The CEA/CD3-Bispecific Antibody MEDI-565 (MT111) Binds a Nonlinear Epitope in the Full-Length but Not a Short Splice Variant of CEA.
|
|
|
|
Optimization of Circulating Biomarkers of Obatoclax-Induced Cell Death in Patients with Small Cell Lung Cancer.
|
|
|
|
A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo.
|
|
|
|
A small molecule inhibitor of XIAP induces apoptosis and synergizes with vinorelbine and cisplatin in NSCLC.
|
|
|
|
|
View All
|
Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters