Scientific Frequently Asked Question

Results 51 - 75 of 87 for FAQ

FAQ	Category
Should I choose a 2-fold or 4-fold dilution for my samples in the V-PLEX TH17 Panel 1 (human)? Sample dilution is dependent on the expected analyte levels in the samples. &nbs...	Assays
Should the control values fall within a certain range of set values? The lot-specific concentration of each analyte in the control pack is provided i...	Assays
To conserve on sample volume, can you use 25 µl of sample in the validated Human Aß42 and Human Total Tau kits instead of 50 µl? We have validated the assays using 50 µl of sample (1:8 or 1:4 diluted). R...	Assays
V-PLEX and V-PLEX Plus Questions Does the V stand for validated? V-PLEX products are MSD's...	Assays
What amount (micrograms) of lysate should I use? The performance of the assay will depend greatly on the abundance of the protein...	Assays
What are the release specifications (QC) for the kits? All of our validated kits come with a Certificate of Analysis (COA) that documen...	Assays
What do I need to purchase along with the R-PLEX and/or U-PLEX Antibody Sets to perform a multiplex assay? Multiplex assays, using either R-PLEX antibody sets or a combination of R-PLEX a...	Assays
What do I need to purchase along with the R-PLEX Antibody Set to perform a singleplex assay? The R-PLEX Antibody Set includes a matched antibody set and calibrator. MSD GOLD...	Assays
What is the difference between an R-PLEX Antibody Set and a U-PLEX Antibody Set? R-PLEX Antibody Sets contain a biotinylated capture antibody, a SULFO-TAG conjug...	Assays
What is the difference between anti-hu IL-8 and IL-8 (HA) detection antibodies? The anti-hu IL-8 (HA) antibody has been validated and is recommended when high I...	Assays
What is the difference between V-PLEX and V-PLEX Plus kits? The V-PLEX Plus kits include additional items (controls, wash buffer, and plate ...	Assays
What is the MSD Lysis Buffer formulation? 150 mM NaCl 20 mM Tris, pH 7.5 1 mM EDTA 1 mM EGTA 1% T...	Assays
What is the sample volume required for the cytokine 30-plex or the 40-plex V-PLEX kits? The sample volume required depends on sample type and dilution factor used. Reco...	Assays
What is the shelf life of the Human Aß42 and Human Total Tau kits? Plates expire 15 months from the date of manufacture. Kit expiration dates are b...	Assays
What is the turnaround time for orders? Catalog Items Our catalog assays generally ship within 1-3 business days. Cust...	Assays Ordering
What other lysis buffers have been used with MSD assays? Various modified RIPA buffers with low concentrations of ionic detergents and ur...	Assays Reagents Diluents Buffers
What parameters can I optimize for my particular sample? End users can vary the following parameters: Concentration of detection a...	Assays
What plates do I purchase for R-PLEX multiplex assays? R-PLEX multiplex assays use 10-spot 96-well U‑PLEX plates, available as U-PLEX D...	Assays
What should I do if I have less than 25 µL of sample? A minimum of 25 µL of solution is required to prevent the plate surface fr...	Assays
What should I do if the read buffer is added to the plate, but the instrument is not available? If the instrument is not available, MSD recommends that you remove the read buff...	Assays Reagents Diluents Buffers
What standards are used to calibrate the assays? The assays are developed using positive and negative control Whole Cell Lysate S...	Assays
What volume of lysate should I use? A minimum of 25 µL should be placed in each well to ensure adequate covera...	Assays
Where can I find general information about MSD assay development and plate spotting? The MSD Immunoblot and General Notes technical presentation includes detailed in...	Assays
Where can I find information about immunogenicity assay development? This product insert provides detailed information about using MSD Bridging ...	Assays
Where can I find the calibrator/standard curve concentrations for V-PLEX assays? A certificate of analysis (COA) is provided with each V-PLEX kit lot including t...	Assays

Results 51 - 75 of 87 for FAQ

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