FAQ | Category | |
Does the Human Total Tau Kit work for mouse samples? We have tested mouse brain lysates with the Human Total Tau Kit and were unable ... | Assays | |
FAQs for V-PLEX Proinflammatory Panel 1 (mouse) Kits
What is the difference between the 10-plex panel, a custom panel, and the s... | Assays | |
How are V-PLEX assays different from T-PLEX assays? Unlike T-PLEX assays (tissue culture, ultra-sensitive, whole cell lysate, serum/... | Assays | |
How can I control cross-reactivity in multiplex assays? MSD has several blockers available that can be added to lysis buffer or antibody... | Assays | |
How can I obtain a Certificate of Analysis (COA)? COAs are available on our website. In order to locate the correct COA for any pr... | Assays | |
How critical are the incubation times when running V-PLEX assays? Are there any steps where I can incubate the samples overnight? For consistent results, MSD recommends keeping incubation times consistent withi... | Assays | |
How did MSD decide which specific kidney biomarkers to target? MSD worked with the Critical Path Institute (C-Path) and the Predictive Safety T... | Assays | |
How do I measure percent phosphorylation? Perform a lysate titration to establish the linear range of the assay. For sampl... | Assays | |
How long should I leave the assay in read buffer before I read the plate? Unless otherwise directed in the protocol, incubation in read buffer is not reco... | Assays Reagents Diluents Buffers | |
How many samples can I run on a plate? MSD assays are provided in convenient 96- or 384-well microtiter plates. The num... | Assays Plates | |
How much secondary antibody do I use for my assay development? For initial assay development, it is recommended that a 1:1 primary:secondary an... | Assays | |
I am multiplexing R-PLEX with U-PLEX analytes. The R PLEX protocol says to dilute the U PLEX calibrators 10 fold into the calibrator blend. But the U PLEX certificate of analysis (COA) provides the calibrator concentrations for a 5-fold dilution. The U-PLEX Calibrators are reconstituted with a lower volume (high concentration... | Assays | |
I have controls that were made in CSF that do not recover between 80% and 120% (or the CVs are greater than 20%). Pooled CSF can have interferences that are not present in properly collected and... | Assays | |
I’m not getting any signal with my R-PLEX assay. Check to ensure that the reagents were added correctly. Make sure that you used ... | Assays | |
I obtained low spike recovery for the osteopontin assay in the MSD Kidney Injury Panel 1 (rat) Kit. Why is that? Osteopontin binds to calcium oxalate. If calcium oxalate crystals are present in... | Assays | |
If I have diluent or detection antibody reagents left over from a previous V-PLEX assay kit, can I use them in another V-PLEX kit? Check the reagent lot numbers, which are found in the certificate of analysis (C... | Assays | |
Is a phospho-specific antibody always used to capture the phosphoprotein? The phospho-specific antibody may be the capture or the detection antibody with ... | Assays | |
Is an MSD instrument required to run MSD assays? Yes. The MSD platform is unique in its method for detection. As a result, MSD in... | Instrument Assays Plates | |
Is it necessary to use MSD GOLD Read Buffer with the R-PLEX singleplex assays? The R-PLEX multiplex assays use Read Buffer T. MSD GOLD Read Buffer is more convenient since it is provided at the typical work... | Assays | |
Is the lower limit of quantitation (LLOQ) in V-PLEX assays based on endogenous levels of the analyte? It is difficult to resolve an LLOQ if the endogenous levels of analytes in norma... | Assays | |
Is the same antibody pair used for V-PLEX kits and/or T-PLEX assays as used for R-PLEX sets for the same analyte? The antibody pairs are not necessarily the same. V-PLEX, U-PLEX, T-PLEX, S-PLEX ... | Assays | |
Is the validated Human Aß42 assay available with 4G8 detection? The Human Aß42 assay was developed and validated with 6E10 detection antib... | Assays | |
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Analysis of Results)
Why do you recommend normalization to total protein mass per well rather th... | Assays | |
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Product info)
Do the new total Akt and phospho-Akt (Ser473) assays use the same antibody... | Assays | |
Should I block the streptavidin plate before coating with capture antibodies? In some cases, blocking the plate prior to coating helps to reduce variability. ... | Assays | |