Th17 U-PLEX Assays

U-PLEX: MSD Launches NEW Th17 Assays

 

Th17: A Pathway Implicated in Immune Disorders

T cells of the Th17 lineage play key roles in immune responses. Th17 cells are a critical part of the immune system and act as a bridge between the innate and adaptive immune systems. Improper regulation of their proinflammatory activities contributes to numerous pathogenic conditions such as rheumatoid arthritis, psoriasis, type 1 diabetes, multiple sclerosis, Crohn’s disease, and asthma.

U-PLEX: MSD’s Most Flexible Assay Platform

The flexibility of the U-PLEX platform empowers you to make personalized multiplex assay combinations of your choice. Now you can build and run your multiplex assay at your convenience without any additional equipment or time-consuming assay development.

Discover new combinations with the quality and performance you expect from MSD.

Design Your Th17 Multiplex


U-PLEX Combinations for Th17 Research

U-PLEX Combinations represent popular combinations of analytes, grouped into separate catalog numbers for ordering convenience.

Species Name Catalog Number Analytes
Human TH17 Combo 1 K15075K IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-31, IL-33
Human TH17 Combo 2 K15076K IFN-γ, IL-1β, IL-6, IL-10, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, TNF-α
NHP TH17 Combo 1 K15079K IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-17A, TNF-α
Mouse TH17 Combo 1 K15077K IL-17A, IL-17C, IL-17E, IL-17F, IL-21, IL-22, IL-23, IL-31, IL-33
Mouse TH17 Combo 2 K15078K IFN-γ, IL-1β, IL-6, IL-17A, IL-17C, IL-17E, IL-17F, IL-21, IL-22, TNF-α

Th17-Related Analytes

Individual analytes are available as singleplex assays that can be configured into customized multiplex combinations of your choice. Combine MSD analytes with your own analytes for a truly custom multiplex assay.

Human
Analyte LLOD – ULOD, pg/mL
IFN-γ 1.7 – 17,000
IL-1β 0.15 – 3,800
IL-6 0.33 – 2,000
IL-10 0.14 – 3,700
IL-17A 2.6 – 23,400
IL-17A/F 1.8 – 18,400
IL-17E/IL-25 0.58 – 9,200
IL-17F 155 – 112,000
IL-21 1.2 – 12,600
IL-22 0.13 – 3,400
IL-23 1.4 – 21,600
IL-27 9.6 – 50,600
IL-31 7.3 – 11,060
IL-33 0.59 – 10,300
TNF-α 0.54 – 3,700
NHP
Analyte LLOD – ULOD, pg/mL
IFN-γ 1.7 – 17,000
IL-1β 0.15 – 3,800
IL-2 0.7 – 1,900
IL-4 0.06 – 2,100
IL-5 0.24 – 4,000
IL-6 0.33 – 2,000
IL-8 0.15 – 2,200
IL-10 0.14 – 3,700
IL-17A 2.3 – 23,400
TNF-α 0.54 – 3,700
Mouse
Analyte LLOD – ULOD, pg/mL
IFN-γ 0.16 – 2,900
IL-1β 3.1 – 13,000
IL-6 4.8 – 16,000
IL-10 3.8 – 22,800
IL-17A 0.30 – 2,100
IL-17C 2.3 – 45,600
IL-17E/IL-25 1.6 –18,900
IL-17F 24 – 52,600
IL-21 6.5 – 40,600
IL-22 1.2 – 1,800
IL-23 4.9 – 20,400
IL-27 8.7 – 73,300
IL-31 45 – 66,300
IL-33 2.2 – 36,100
TNF-α 1.3 – 6,200

Additional analytes are available on the U-PLEX platform. View our complete portfolio here.


Calibration Curves and Limits of Detection

The following figures demonstrate typical calibration curves for 12 Th17 related (human) assays. Up to ten markers can be combined per plate to create the desired multiplex.

U-PLEX curve figure 2


The following table demonstrates typical data for the 12 assays tested. Data include Hill slopes and lower limits of detection (LLOD) for the calibration curves. The calibrator is reconstituted and diluted serially (4-fold) to generate a 7-point standard curve.

Average Hill slope and LLOD
Assay IL-17A/F IL-17A IL-17E/
IL-25
IL-17F IL-21 IL-22 IL-23 IL-27 IL-29/
IFN-λ1
IL-31 IL-33 TSLP
Hill slope 1.04 1.00 0.99 1.12 1.00 0.99 0.99 1.04 1.05 1.37 1.03 1.00
LLOD (pg/mL) 2.24 0.89 0.53 118 0.83 0.16 1.16 11.5 1.63 15.6 0.82 0.29

For additional data, click here to read the entire poster.
Development and Characterization of Th17 Related U-PLEX Assays