V-PLEX Plus Human IL-27 Kit


V-PLEX Plus Human IL-27 Kit Overview
The V-PLEX Plus Human IL-27 Kit is optimized for long-term and multi-site biomarker studies that require reliable, reproducible results with lot-to-lot consistency. The V-PLEX Plus Human IL-27 Kit enables the sensitive detection of native IL-27 in multiple sample types including serum, plasma, urine, cell culture supernatants, and PBMCs. To learn more about the advantages of V-PLEX assays click here.
The V-PLEX Plus Human IL-27 assay is available as an individual assay kit as well as in a multiplex combination with other TH17-related analytes in the V-PLEX TH17 Panel 1 (human) Kit (catalog # K15085G, K15085D).
All V-PLEX kits come with coated plates, calibrators, labeled detection antibodies, optimized diluents, a comprehensive product insert, and a certificate of analysis. V-PLEX Plus kits also include controls, wash buffer, and plate seals for added quality and convenience.

V-PLEX Plus Human IL-27 Assay Features

  • Validated to measure human IL-27 in serum, plasma, cell culture supernatants, PBMCs, and urine
  • Dynamic range: 4.20 pg/mL – 13,000 pg/mL
  • LLOQ: 38.7 pg/mL

Typical Data
Calibration curve accuracy and precision for the V-PLEX Plus Human IL-27 assay were assessed and representative data are presented below.

Figure 1. V-PLEX Plus Human IL-27 Calibration Curve
Human IL-27 Calibrator Curve
Figure 2. V-PLEX Plus Human IL-27 Data
Human IL-27 Calibrator Curve Data
IL-27 Analyte

Description - Interleukin-27 (IL-27), a heterodimeric cytokine and a relatively new member of the IL-6/IL-12 family, plays an important role in the regulation of Th1 responses. IL-27 consists of two subunits, an EBV-transformed gene 3 (EBI3) subunit and a p28 cytokine subunit. IL-27 activity is mediated by binding to its receptor, IL-27R, comprised of WSX-1 and gp130. Several immune cells co-express both subunits of the IL-27 receptor. IL-27 is expressed by antigen-presenting cells upon antigen activation. IL-27 potently induces the proliferation of naive T cells, and synergistically with IL-12 stimulates the production of IFN-γ by naive CD4+ T cells.

Disease Focus - Cancer, inflammatory bowel disease, tuberculosis, sarcoidosis, colitis, and Crohn’s disease.

Application Area - Inflammation, cytokines and chemokines

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1 plate
5 plates
25 plates
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