U-PLEX Mouse IL-17E/IL-25 Assay Overview
The U-PLEX Mouse IL-17E/IL-25 Assay maximizes your flexibility to design an experiment that measures IL-17E alone or multiplexed with MSD U-PLEX assays, your own antibody pairs, or a combination of both. U-PLEX assays demonstrate high sensitivity, excellent precision, and up to 5-logs of linear dynamic range. Plates are read in less than 90 seconds on any MSD instrument, using no complicated fluidics or calibration procedures.
U-PLEX Mouse IL-17E/IL-25 Assay Features
- Simple protocol similar to ELISAs, yet uses less sample
- Measures mouse IL-17E in serum, plasma, and cell culture supernatants
- Create custom multiplexes using a selection of U-PLEX assays, your own antibody pairs or both
Assay Format
- Singleplex – includes MSD GOLD Small Spot Streptavidin Plates
- Multiplex – includes U-PLEX multiplex plates
- Both formats use the same assay components for efficient transfer between single- and multiplex configurations.
IL-17E Analyte
Description- Mouse IL-17E (also known as IL-25) is one of 6 homologically related peptides in the IL-17 family. It is a mediator of allergic reactions and protects against intestinal parasites including helminths. IL-17E is upregulated in epithelial cells, TH2 cells, eosinophils and basophils and may contribute to chronic inflammatory responses. It activates NF-κB and stimulates IL-8 production. This is in contrast to other IL-17 family members that promote TH1 and TH17-like responses.
Disease Focus - Allergic reactions including allergic asthma, airway hyperreactivity, elevated IgE levels, intestinal parasites, atopic dermatitis.
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Specifications
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Kit Contents
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Documentation
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References
Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-17E/IL-25
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Species
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Mouse
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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U-PLEX Group Compatibility
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Mouse Biomarker Group 1
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Plate Type
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96-well
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Capture Antibody
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Rat Monoclonal
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Detection Antibody
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Goat Polyclonal
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LLOD (Sensitivity)
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1.6 pg/mL
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Dynamic Range
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1.6-18,900 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant
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Recombinant standards
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Expressed in E. coli
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each (1 plate size)
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2-8°C
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1 vial (1 vial)
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2-8°C
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1 each (1 plate)
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2-8°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (10 mL)
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≤-10°C
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1 bottle (8 mL)
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RT
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1 bottle (18 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each (5 plate size)
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2-8°C
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5 vial (1 vial)
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2-8°C
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5 each (1 plate)
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2-8°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (40 mL)
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RT
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1 bottle (90 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 each (5 plate size)
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2-8°C
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25 vial (1 vial)
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2-8°C
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25 each (1 plate)
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2-8°C
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5 bottle (50 mL)
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≤-10°C
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5 bottle (50 mL)
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≤-10°C
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5 bottle (40 mL)
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RT
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5 bottle (90 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Alopecia areata is characterized by dysregulation in systemic type 17 and type 2 cytokines, which may contribute to disease-associated psychological morbidity
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