U-PLEX Mouse IL-17E/IL-25 Assay Overview
The U-PLEX Mouse IL-17E/IL-25 Assay maximizes your flexibility to design an experiment that measures IL-17E alone or multiplexed with MSD U-PLEX assays, your own antibody pairs, or a combination of both. U-PLEX assays demonstrate high sensitivity, excellent precision, and up to 5-logs of linear dynamic range. Plates are read in less than 90 seconds on any MSD instrument, using no complicated fluidics or calibration procedures.

U-PLEX Mouse IL-17E/IL-25 Assay Features

• Simple protocol similar to ELISAs, yet uses less sample
• Measures mouse IL-17E in serum, plasma, and cell culture supernatants
• Create custom multiplexes using a selection of U-PLEX assays, your own antibody pairs or both

Assay Format

• Singleplex – includes MSD GOLD Small Spot Streptavidin Plates
• Multiplex – includes U-PLEX multiplex plates
• Both formats use the same assay components for efficient transfer between single- and multiplex configurations.

IL-17E Analyte
Description- Mouse IL-17E (also known as IL-25) is one of 6 homologically related peptides in the IL-17 family. It is a mediator of allergic reactions and protects against intestinal parasites including helminths. IL-17E is upregulated in epithelial cells, TH2 cells, eosinophils and basophils and may contribute to chronic inflammatory responses. It activates NF-κB and stimulates IL-8 production. This is in contrast to other IL-17 family members that promote TH1 and TH17-like responses.

Disease Focus - Allergic reactions including allergic asthma, airway hyperreactivity, elevated IgE levels, intestinal parasites, atopic dermatitis.