R-PLEX Human Granzyme B Antibody Set Overview
The R-PLEX Human Granzyme B Antibody Set includes a matched capture and detection antibody pair and calibrator for the quick and easy measurement of Granzyme B in biological samples. R-PLEX products leverage MSD’s electrochemiluminescence technology for better sensitivity and a larger dynamic range than traditional ELISAs. R-PLEX matched antibody sets enable the development of singleplex and multiplex immunoassays. Individual assays may be developed using MSD avidin- or streptavidin-coated plates. Custom multiplex assays with any combination of R-PLEX analytes, as well as R-PLEX and U-PLEX analytes, can be developed on U-PLEX MULTI-SPOT plates. To learn more about the advantages of R-PLEX products
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R-PLEX Human Granzyme B Antibody Set Features
- Measures human Granzyme B in serum, plasma, and cell lysate
- Dynamic range: 0.30-2,000 pg/mL
- Median LLOD: 0.30 pg/mL
- Can be used to develop individual analyte assays as well as multiplex panels
Typical Data
Calibration curve for the R-PLEX Human Granzyme B Antibody Set was assessed and representative data is presented below.
Figure 1. R-PLEX Human Granzyme B Antibody Set Calibration Curve
Granzyme B Analyte
Description - Granzyme B (GZMB, C11, CTLA-1, CTSGL1, SECT) is a protease found in secretory granules of cytotoxic T cells and NK cells. When secreted into the target cell, Granzyme B causes cell death by activating many of the caspase enzymes present inside cells. Granzyme B has other functions including stimulating the release of proinflammatory cytokines and remodeling of extracellular matrices. It may also play a role in the development of chronic obstructive pulmonary disease and hair loss.
Disease Research Area - Infectious Diseases
Application Area - Immunology/Inflammation, Immuno-Oncology
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Recommended Components
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Specifications
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Kit Contents
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Documentation
You will need plates, diluents, and read buffer to run your assay. Please select from the below menu.
R-PLEX Sets are intended to be used with other MSD components such as plates, diluents and MSD Read Buffer. If you don’t already have these items, you can purchase them along with your Antibody Set(s). The “Recommended Components” tab provides helpful suggestions or you can call MSD Customer Service (Phone number) for assistance. R-PLEX Sets can also be used with other antibody sets such as U-PLEX antibody sets (make a link to UPLEX landing page) for multiplex measurements.
Catalog No.
Product Name
Size
R51BB-4
R50AA-4
R92TG-3
L45SA-1
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Application(s)
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Immunology/Inflammation
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Immunogenicity
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Analyte(s)
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Granzyme B
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Species
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Human
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Goat Polyclonal
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Dynamic Range
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0.30-2,000 pg/mL
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Sample Type
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Serum, Plasma, Cell Lysate
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LLOD (Sensitivity)
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0.30 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Gene ID(s)
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3002
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UniProt ID(s)
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P10144
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Assay Diluent(s)
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Diluent 2
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Antibody Diluent(s)
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Diluent 3
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Product Description
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Storage
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Quantity per Kit
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Human Granzyme B Antibody Set (5 Plate Size)
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2-8°C
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1 ea
(5 plate size)
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Human Granzyme B Calibrator
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≤-70°C
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5 vial
(1 vial)
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Product Description
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Storage
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Quantity per Kit
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Human Granzyme B Antibody Set (5 Plate Size)
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2-8°C
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10 ea
(5 plate size)
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Human Granzyme B Calibrator
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≤-70°C
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50 vial
(1 vial)
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Product Inserts
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SDS
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Handouts
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Datasheets
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters