PARP (cleaved) Whole Cell Lysate Kit

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Poly (ADP-ribose) polymerase (PARP) is a nuclear enzyme activated by DNA damage and was one of the first identified death subsrates for caspase-3 in apoptosis. Active PARP transfers ADP-ribose from NAD+ to nuclear proteins, and this modification has been implicated in many fundamental processes like DNA repair, chromatin stability, cell proliferation, and cell death. PARP becomes inactive following cleavage, however the small cleavage product does retain its DNA-binding capabilities, thereby blocking access to damaged DNA and inhibiting further DNA repair. This kit provides assay-specific components for the quantitative determination of PARP (cleaved) (Asp214) in human whole cell lysates.

Please note: MSD has discontinued this product.

Specifications
Documentation
References

Application(s)		Phosphoproteins , Intracellular Signaling
Analyte(s)		PARP
Species		Human
Instrument(s)		SECTOR Imager 2400 , MESO SECTOR S 600MM , SECTOR Imager 6000 , MESO QuickPlex SQ 120MM , MESO QuickPlex SQ 120 , MESO SECTOR S 600
Plate Type		96-well
Capture Antibody		Rabbit Polyclonal
Detection Antibody		Rabbit Polyclonal
Analyte State		Cleaved
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Kit Model		Whole Cell Lysate
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

5:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		Cleaved PARP WCL
Datasheets		DS Cleaved PARP
SDS		Blocker A Protease Inhibitor Solution Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) Tris Lysis Buffer Tris Wash Buffer (10x)

Title	Journal	Year
Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233. Gurley SN, Abidi AH, Allison P, Guan P, Duntsch C, Robertson JH, Kosanke SD, Keir ST, Bigner DD, Elberger AJ, Moore BM 2nd.	J Neurooncol.	2012
High sensitivity of BRCA1-deficient mammary tumors to the PARP inhibitor AZD2281 alone and in combination with platinum drugs. Rottenberg, S., Jaspers, J.E., Kersbergen, A., van der Burg, E., Nygren, A.O., Zander, S.A., Derksen, P.W., de Bruin, M., Zevenhoven, J., Lau, A., Boulter, R., Cranston, A., O'Connor, M.J., Martin, N.M., Borst, P., Jonkers, J.	PNAS	2008
Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen. Sasiela, C.A., Stewart, D.H., Kitagaki, J., Safiran, Y.J., Yang, Y., Weissman, A.M., Oberoi, P., Davydov, I.V., Goncharova, E., Beutler, J.A., McMahon, J.B., O'Keefe, B.R.	J Biomol Screen.	2008
Anticancer Activity of BIM-46174, a New Inhibitor of the Heterotrimeric Ga/Gßg Protein Complex. Prevost, G.P., Lonchampt, M.O., Holbeck, S., Attoub, S., Zaharevitz, D., Alley, M., Wright, J., Brezak, M.C., Coulomb, H., Savola, A., Huchet, M., Chaumeron, S., Nguyen, Q.D., Forgez, P., Bruyneel, E., Bracke, M., Ferrandis, E., Roubert, P., Demarquay, D., Gespach, C., Kasprzyk, P.G.	Cancer Res.	2006

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PARP (cleaved) Whole Cell Lysate Kit

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