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The Human ProInflammatory 9-Plex Base Kit provides basic components for the simultaneous quantitative determination of both natural and recombinant human GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-10, IL-12p70, and TNF-α. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.
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Specifications
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Documentation
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References
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-10, IL-12p70, TNF-α
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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GM-CSF: Mouse Monoclonal
IFN-γ: Mouse Monoclonal
IL-1β: Mouse Monoclonal
IL-2: Mouse Monoclonal
IL-6: Mouse Monoclonal
IL-8: Mouse Monoclonal
IL-10: Rat Monoclonal
IL-12p70: Mouse Monoclonal
TNF-α: Mouse Monoclonal
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Detection Antibody
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GM-CSF: Rat Monoclonal
IFN-γ: Mouse Monoclonal
IL-1β: Goat Polyclonal
IL-2: Mouse Monoclonal
IL-6: Goat Polyclonal
IL-8: Goat Polyclonal
IL-10: Rat Monoclonal
IL-12p70: Mouse Monoclonal
TNF-α: Goat Polyclonal
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LLOD (Sensitivity)
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GM-CSF: 0.34 pg/mL
IFN-γ: 0.53 pg/mL
IL-1β: 0.36 pg/mL
IL-2: 0.35 pg/mL
IL-6: 0.27 pg/mL
IL-8: 0.12 pg/mL
IL-10: 0.23 pg/mL
IL-12p70: 1.39 pg/mL
TNF-α: 0.50 pg/mL
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Dynamic Range
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GM-CSF: 0.34-10000 pg/mL
IFN-γ: 0.53-10000 pg/mL
IL-1β: 0.36-10000 pg/mL
IL-2: 0.35-10000 pg/mL
IL-6: 0.27-10000 pg/mL
IL-8: 0.12-10000 pg/mL
IL-10: 0.23-10000 pg/mL
IL-12p70: 1.39-10000 pg/mL
TNF-α: 0.50-10000 pg/mL
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Sample Type
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Cell Culture Supernatant
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Recombinant standards
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GM-CSF, IFN-γ, IL-1β, IL-2, IL-6, IL-8, TNF-α: Recombinant protein expressed in E. coli
IL-10, IL-12p70: Expressed in insect cells
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8 °C
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Title
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Journal
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Year
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Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke
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A phase 1 study of prophylactic anakinra to mitigate ICANS in patients with large B-cell lymphoma
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Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms
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Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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Scientific/Technical Support
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