Activation of the PI-3 kinase pathway results in the translocation of Akt to the plasma membrane and its subsequent phosphorylation at Ser473 by 3-phosphoinositide-dependent kinase-1 (PDK1) or other kinases. Activated Akt then phosphorylates a number of downstream targets regulating cell growth, cell survival, cancer, and diabetes. This kit provides basic components for the quantitative determination of phospho-Akt (Ser473) in human, mouse, and rat whole cell lysates. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components.) Base kits are most commonly used for high-throughput screening and assay development.