A role for reactive oxygen species in endotoxin-induced elevation of MOR expression in the nervous and immune systems.

Langsdorf, E.F., Mao, X., Chang, S.L.

Journal	J Neuroimmunol.		Year	2011
Species	Human		Volume	236
Analytes Measured	GM-CSF, IFN-γ, IL-1β, IL-10, IL-12 p70, IL-2, IL-6, IL-8, TNF-α		Page #	57-64
Matrix Tested	Cell culture supernatants		Category	Cytokines & Chemokines

---

Abstract

This study examined the mechanism by which exposure to lipopolysaccharide (LPS) alters mu-opioid receptor (MOR) expression in immune and neuronal cells using an in vitro conditioned medium model system. We found that LPS stimulated the intracellular accumulation of reactive oxygen species (ROS) and MOR expression in macrophage-like TPA-HL-60 cells. Conditioned medium from the LPS-stimulated TPA-HL-60 cells increased MOR expression in SH-SY5Y cells, a neuronal cell model, through actions mediated by TNF-α and GM-CSF. These data suggest that the endotoxin, LPS, modulates MOR expression in nervous and immune cells via ROS signaling, and demonstrates the crosstalk that exists within the neuroimmune axis.

Related Products

Human Chemokine 7-Plex Ultra-Sensitive Kit

Human Chemokine 9-Plex Base Kit

Human Chemokine 9-Plex Tissue Culture Kit

Human Chemokine 9-Plex Ultra-Sensitive Kit

Human Demonstration 10-Plex Base Kit

Human Demonstration 10-Plex Tissue Culture Kit

Human Demonstration 4-Plex Base Kit

Human Demonstration 4-Plex Tissue Culture Kit

Human Demonstration 7-Plex Base Kit

Human Demonstration 7-Plex Tissue Culture Kit

Human ProInflammatory 7-Plex Base Kit

Human ProInflammatory 7-Plex Tissue Culture Kit

Human ProInflammatory 7-Plex Ultra-Sensitive Kit

Human ProInflammatory 9-Plex Base Kit

Human ProInflammatory 9-Plex Tissue Culture Kit

Human ProInflammatory 9-Plex Ultra-Sensitive Kit

Human ProInflammatory-4 I Base Kit

Human ProInflammatory-4 I Tissue Culture Kit

Human ProInflammatory-4 I Ultra-Sensitive Kit

Human ProInflammatory-4 II Base Kit

Human ProInflammatory-4 II Tissue Culture Kit

Human ProInflammatory-4 II Ultra-Sensitive Kit

Human TH1/TH2 10-Plex Base Kit

Human TH1/TH2 10-Plex Tissue Culture Kit

Human TH1/TH2 10-Plex Ultra-Sensitive Kit

Human TH1/TH2 7-Plex Base Kit

Human TH1/TH2 7-Plex Tissue Culture Kit

Human TH1/TH2 7-Plex Ultra-Sensitive Kit

MS6000 Hu ProInflammatory-4 II 384 Tissue Culture Kit

Human GM-CSF 384 Tissue Culture Kit

Human GM-CSF Base Kit

Human GM-CSF Tissue Culture Kit

Human GM-CSF Ultra-Sensitive Kit

Human IFN-gamma 384 Tissue Culture Kit

Human IFN-gamma Base Kit

Human IFN-gamma Tissue Culture Kit

Human IFN-gamma Ultra-Sensitive Kit

Human IL-10 384 Tissue Culture Kit

Human IL-10 Base Kit

Human IL-10 Tissue Culture Kit

Human IL-10 Ultra-Sensitive Kit

Human IL-12p70 384 Tissue Culture Kit

Human IL-12p70 Base Kit

Human IL-12p70 Tissue Culture Kit

Human IL-12p70 Ultra-Sensitive Kit

Human IL-1 beta 384 Tissue Culture Kit

Human IL-1 beta Base Kit

Human IL-1 beta Tissue Culture Kit

Human IL-1 beta Ultra-Sensitive Kit

Human IL-2 384 Tissue Culture Kit

Human IL-2 Base Kit

Human IL-2 Tissue Culture Kit

Human IL-2 Ultra-Sensitive Kit

Human IL-6 384 Tissue Culture Kit

Human IL-6 Base Kit

Human IL-6 Tissue Culture Kit

Human IL-6 Ultra-Sensitive Kit

Human IL-8 384 Tissue Culture Kit

Human IL-8 Base Kit

Human IL-8 Tissue Culture Kit

Human IL-8 Ultra-Sensitive Kit

Human TNF-alpha 384 Tissue Culture Kit

Human TNF-alpha Base Kit

Human TNF-alpha Tissue Culture Kit

Human TNF-alpha Ultra-Sensitive Kit

MA6000 Human GM-CSF 384 Tissue Culture Kit

MA6000 Human IFN-g 384 Tissue Culture Kit

MA6000 Human IL-10 384 Tissue Culture Kit

MA6000 Human IL-12p70 384 Tissue Culture Kit

MA6000 Human IL-1b 384 Tissue Culture Kit

MA6000 Human IL-2 384 Tissue Culture Kit

MA6000 Human IL-6 384 Tissue Culture Kit

MA6000 Human IL-8 384 Tissue Culture Kit

MA6000 Human TNF-a 384 Tissue Culture Kit

view more