General and Assay Development

Q: What is the difference between read buffers? A: MSD® Read Buffers differ in their chemical formulations, allowing one (or more) buffers to be better suited to a specific assay application. The MSD Read Buffer Guide provides a description of each buffer and highlights its useful applications. Q: How does one read buffer out perform others in applications? A: The formulation of some buffers facilitates better results in either washed or non-washed assay formats or in specific types of applications. The MSD Read Buffer Guide provides detailed information on choosing the correct Read Buffer for your application. Q: How much secondary antibody do I use for my assay development? A: For initial assay development, it is recommended that a 1:1 primary:secondary antibody ratio be used. For example, 1 µg/mL primary detection antibody: 1 µg/mL secondary detection antibody. A recommended starting concentration for each antibody is 1 µg/mL (6.7 nM), and can be adjusted according to initial assay results. Q: What is the binding capacity of avidin and streptavidin plates? A: For Standard plates, the avidin or streptavidin-coated electrode surface is capable of binding 0.3 picomole of capture antibody from solution. For High-Bind plates, the avidin or streptavidin-coated electrode surface is capable of binding 0.6 picomoles of capture antibody from solution. Q: How do I read a plate? A: To read, preserve and store a partial plate, please follow the steps in this document. Q: How do I read a partial plate? A: To read, preserve and store a partial plate, please follow the steps in this document. Q: Where can I find information about immunogenicity assay development? A: This product insert provides detailed information about using MSD Bridging Immunogenicity Assays. Q: Where can I find general information about MSD assay development and plate spotting? A: The MSD Immunoblot and General Notes technical presentation includes detailed information about MSD Immuno-Dot-Blot assays and general MSD assay development topics including plate spotting, appropriate assay volumes, plate binding capacity, appropriate signal levels, running a partial plate, blocking, and read buffers. Q: What are the dimensions of your 96- and 384-well plates? A: 96-Well Plate Parameters Measurement Overall Footprint 3.365 in. x 5.030 in. Overall Height 0.565 in. Well Spacing (Center-to-Center) 0.3543 in. (9 mm) Well Dimensions at Top 0.276 in. x 0.276 in. Well Dimensions at Bottom 0.252 in. x 0.252 in. Well Depth 0.460 in. Total Well Volume 410 µl Bottom of Wells to Resting Plane of Plate 0.105 in. 384-Well Plate Parameters Measurement Overall Footprint 3.365 in. x 5.030 in. Overall Height 0.400 in. Well Spacing (Center-to-Center) 0.1772 in. (4.5 mm) Well Dimensions at Top 0.1472 in. x 0.1472 in. Well Dimensions at Bottom 0.1222 in. x 0.1222 in. Well Depth 0.295 in. Total Well Volume 86 µl Bottom of Wells to Resting Plane of Plate 0.105 in. Well pattern centerline and footprint centerline are coincident.