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The Rat Demonstration 7-Plex Base Kit provides basic components for the simultaneous quantitative determination of both natural and recombinant rat IFN-γ, IL- 1β, IL-4, IL-5, IL-13, KC/GRO/CINC, and TNF-α in tissue culture supernatant. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.
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Specifications
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Documentation
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References
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IFN-γ, IL-1β, IL-4, IL-5, IL-13, KC/GRO, TNF-α
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Species
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Rat
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Plate Type
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96-well
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Instrument(s)
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MESO SECTOR S 600MM
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MESO QuickPlex SQ 120MM
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MESO SECTOR S 600
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MESO QuickPlex SQ 120
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SECTOR Imager 6000
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SECTOR Imager 2400
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Capture Antibody
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IFN-γ: Mouse Monoclonal
IL-1β: Mouse Monoclonal
IL-4: Mouse Monoclonal
IL-5: Rat Monoclonal
KC/GRO: Rabbit Polyclonal
IL-13: Goat Polyclonal
TNF-α: Hamster Monoclonal
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Detection Antibody
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IFN-γ: Goat Polyclonal
IL-1β: Goat Polyclonal
IL-4: Goat Polyclonal
IL-5: Rat Monoclonal
KC/GRO: Goat Polyclonal
IL-13: Goat Polyclonal
TNF-α: Goat Polyclonal
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LLOD (Sensitivity)
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IFN-γ: 9.0 pg/mL
IL-1β: 25 pg/mL
IL-4: 1.6 pg/mL
IL-5: 25 pg/mL
KC/GRO: 8.3 pg/mL
IL-13: 3.4 pg/mL
TNF-α: 54 pg/mL
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Dynamic Range
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IFN-γ: 9.0-40000 pg/mL
IL-1β: 25-40000 pg/mL
IL-4: 1.6-40000 pg/mL
IL-5: 25-40000 pg/mL
KC/GRO: 8.3-40000 pg/mL
IL-13: 3.4-40000 pg/mL
TNF-α: 54-40000 pg/mL
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Sample Type
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Serum, Plasma
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8 °C
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Title
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Journal
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Year
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A phase 1 study of prophylactic anakinra to mitigate ICANS in patients with large B-cell lymphoma
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Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke
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Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms
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Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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