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Enter author, analyte, or keyword to search publications.
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Miao, W., Ge, C. "Electrogenerated Chemiluminescence (ECL)". Encyclopedia of Supramolecular Chemistry (2010).
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| Tian-Yu, J., Licht, S., Pardee, G., Bhat, A., Cao, Y., Gao, W., Sangalang, E., Zaror, I. (2010) Binding Rate Screen - A high-throughput assay in soluble lysate for prioritizing protein expression constructs. Anal Biochem. Vol. 399(2):276-283.
High throughput, biosensor, ForteBio, Octet
Analytes tested using MSD® assays: Expressed monomeric recombinant protein in cell lysate
Matrix tested: Cell lysate
Summary: In this paper, the authors developed a Binding Rate Screen (BRS) to select and prioritize recombinant tagged protein constructs as expressed in crude soluble lysate (CSL) by measuring the rates at which they bind to a tag-specific antibody and use this as a metric to reflect aggregation, concentration and purifiability.
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| Alexopoulos, L.G., Saez-Rodriguez, J., Espelin, C.W. (2009) High-Throughput Protein-Based Technologies and Computational Models for Drug Development, Efficacy, and Toxicity in Drug Efficacy, Safety, and Biologics Discovery: Emerging Technologies and Tools. Ed. Ekins, S. Wiley InterScience.
protein microarray, ELISA, MESO SCALE DISCOVERY, xMAP technology, Luminex, reverse phase assay, In-cell Western, LICOR, Flow cytometry, genomics, transcriptomics, metabolomics, proteomics
Summary: This book chapter reviews the different high throughput technologies and their roles in the area of drug development, efficacy, and toxicity. Experimental platforms for protein measurements, multiplex technologies, data analysis, modeling and computation have been discussed in detail.
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| Rhyne, P.W., Wong, O.T., Zhang, Y.J., Weiner, R.S. (2009) Electrochemiluminescence in Bioanalysis. Bioanalysis Vol. 1(5):919-935.
ORIGEN Analyzer, BioVeris Analyzer, MESO SCALE DISCOVERY, multiplexing, capillary electrophoresis, hook effect
Summary: This is a review article that discusses the electrochemiluminescence technology and its principles in detail, as well as the applications of ECL-based assays in different fields including microbiology, immunology, neurodegenerative diseases, autoimmunity, cancer, infectious diseases, biowarfare and drug development. The advantages of ECL assays have been mentioned and examples have been provided.
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| Sourial, S., Marcusson-Stahl, M., Cederbrant, K. (2009) Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K. J Biomed Biotechnol. Vol. 2009:187426.
renal damage, distal tubule, technology comparisons
Analytes tested using MSD assays: Calbindin D28K
Matrix tested: Human and rat kidney homogenates and urine
Summary: In this study, the authors developed and compared an immunoassay for calbindin D28K on two multiplex platforms, Luminex and MESO SCALE DISCOVERY®, using the same antibody pair and with rat recombinant calbindin D28K protein as a standard.
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| Christensen, E., Evans, K.R., Menard, C., Pintilie, M., Bristow, R.G. (2008) Practical approaches to proteomic biomarkers within prostate cancer radiotherapy trials. Cancer Metastasis Rev. Vol. 27(3):375-85.
Radiotherapy, clinical trial, mass spectrometry, Luminex, XMAP, MESO SCALE DISCOVERY, ELISA, array
Summary: In this study, the authors investigated the proteomic signatures in immediate risk prostrate cancer patients receiving radiation therapy using multiplexed immunoassays. The importance of specimen acquisition, preparation and analysis, as well as the practical approaches to longitudinal biomarker studies has been discussed.
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| Chung, J., Lee, S., Kris, Y., Braunschweig, T., Traicoff, J., Hewitt, S. (2008) A well-based reverse-phase protein array applicable to extracts from formalin-fixed paraffin-embedded tissue. Proteomics Clin Appl. Vol. 2 (10-11):1539-1547.
Western blot, formalin fixed paraffin embedded tissue, Laser capture microdissection, Protein extraction, reverse phase protein array
Analytes tested using MSD assays: Prostrate specific antigen (PSA), GAPDH, smooth muscle actin
Matrix tested: Protein extracted from FFPE tissue
Summary: This paper discusses a new method for the optimization of protein extraction from formalin-fixed, paraffin embedded tissues to gain high quality intact protein for further analysis of biomarkers.
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| Daniels, D. Applicability of Biomarkers Is Far-Reaching. (2008) Gen Eng Biotech News. Vol. 28(14).
Pronota, NextGen Sciences
Summary: In this news article, the latest techniques used to advance biomarker research have been discussed. miRNA technique from Luminex, Ziplex Microarray Workstation from Xceed Molecular, UNIarray program from Protagen, biomarker development by Biotrin International, use of MESO SCALE DISCOVERY assays by Merck, reverse-phase protein microarray method from Theranostics Health, and several contract research organizations have been highlighted.
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| Dieterle, F., Marrer, E. (2008) New technologies around biomarkers and their interplay with drug development. Anal Bioanal Chem. Vol. 390(1):141-54.
MESO SCALE DISCOVERY, Luminex, VeraCode
Summary: This is a review paper that provides a critical examination of recently established platforms and technologies for biomarker development, and their applications from discovery to validation in genetics, genomics, proteomics, metabonomics and assay development.
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| Gerszten, R.E., Accurso, F., Bernard, G.R., Caprioli, R.M., Klee, E.W., Klee, G.G., Kullo, I., Laguna, T.A., Roth, F.P., Sabatine, M., Srinivas, P., Wang, T.J., Ware, L.B. (2008) Challenges in translating plasma proteomics from bench to bedside: update from the NHLBI Clinical Proteomics Programs. Am J Physiol Lung Cell Mol Physiol. Vol. 295(1):L16-22.
proteome
Summary: This paper discusses the highlights of a meeting held by the National Heart, Lung, and Blood Institute’s (NHLBI) Clinical Proteomics Program committee which focused on the barriers to achieving effective translation of plasma protein biomarkers from discovery to clinical use, and the strategies for overcoming these challenges. The strengths and limitations of multiplexing platforms, including planar assays, bead-based assays and mass spectrometry, have been reviewed.
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| Jia, Y., Gu, X., Brinker, A., Warmuth, M. (2008) Measuring the tyrosine kinase activity: a review of biochemical and cellular assay technologies. Expert Opinion on Drug Discovery. Vol. 3(8) 959-978.
SPA, FlashPlate, ELISA, DELFIA, homogeneous time resolved fluorescence (HTRF), lanthanide chelate excitation, LANCE, Fluorescence polarization, AlphaScreen, Immobilized metal ion affinity partitioning, IMAP, Caliper technology, Z’-Lyte, Easylite, Kinase-Glo, PKLight, ADP Quest, MSD, SureFire, fluorescence quenching assay, IQ, Lightspeed, TruLight
Summary: This review provides a discussion on the different biochemical and cellular kinase assay technologies that are available, their principles, and their application in tyrosine kinase drug discovery. Radiometric assays, Scintillation proximity assays, fluorescence based assays, cell-based kinase assays, translocation assays, kinase sensor assays, and distal assays have been described here.
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Miao, W. (2008) Electrogenerated Chemiluminescence and Its Biorelated Applications. Chem Rev. Vol. 108(7):2506–2553.
Summary: This review paper discusses the fundamentals of electrochemiluminescence (ECL) technology, its principles and unique features with respect to other luminescence, mechanisms of different ECL systems, new developments, instrumentation, luminophores, and the various applications of ECL, especially in biological field.
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| van Oostrum J, Voshol. H. (2008) Antibody based Proteomics to Study Cellular Signaling Networks. Europ Pharma Review. Vol. 13(2):31-35.
LICOR, MESO SCALE DISCOVERY, Luminex, BioPlex, ZeptoChips, BD Phosflow
Summary: In this review, the authors discussed the different tools that are available for the analysis of phosphoproteins, including Western blotting, in-cell Western, solid phase immunoassays, bead based immunoassays, reverse phase assays, and flow cytometry.
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| Wingren, C., Borrebaeck, C.A.K. Protein Microarray Technologies for Detection and Identification of Bacterial and Protein Analytes In Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems. ed. Zourob, M. et al. p715-729. Springer, New York. 2008.
proteomics, antibody microarray, reverse phase protein array
Summary: This paper discusses the potential and specific use of functional protein microarrays and affinity protein microarrays for the detection and serotyping of bacteria, detection of bacterial proteins and toxins, and the identification of diagnostic biomarkers.
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| Blatny, J.M.. (2007) Detecting and Responding to Bioterrorism (Chapter) in Risk Assessment and Risk Communication Strategies in Bioterrorism Preparedness. ed. Green, M.S. et al. Springer Netherlands.
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Kadkhodayan, S., Elliott, L.O., Mausisa, G., Wallweber, H.A., Deshayes, K., Feng, B., Fairbrother, W.J. (2007) Evaluation of assay technologies for the identification of protein-Peptide interaction antagonists. Assay Drug Dev Technol. Vol. 5(4):501-13.
Summary: In this study, the authors compared several assay detection technologies that are routinely used in drug discovery. Multiple peptides were evaluated to develop a small molecule antagonist to the melanoma inhibitor of apoptosis protein using different assays, including ELISA, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), electrochemiluminescence assay (BioVeris), amplified luminescent proximity homogeneous assay (AlphaScreen), time-resolved fluorescence resonance energy transfer (TR-FRET), and fluorescence polarization (FP).
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| Pyati, R., Richter, M.M. (2007) ECL—Electrochemical luminescence. Annu. Rep. Prog. Chem., Sect. C: Phys. Chem., Vol. 103:12-78.
DPA, tris-2,2’-bipyridylruthenium, High performance liquid chromatography (HPLC), capillary electrophoresis, BioVeris ORIGEN and M-SERIES, Roche Elecsys and MODULAR, NucliSens, Eisai PicoLumi, MESO SCALE DISCOVERY SECTOR
Summary: This paper provides a detailed review on the principles, luminophores (diphenylanthracene or Ru(bpy)32+)and applications of electrochemiluminescence technology.
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| Sarker, D., Pacey, S., Workman, P. (2007) Use of pharmacokinetic/ pharmacodynamic biomarkers to support rational cancer drug development. Biomarkers in Medicine. Vol. 1(3): 399-417.
cancer, antineoplastic agents, neoplasm
Summary: This is a review paper that discusses the importance and the use of pharmacokinetic and pharmacodynamic biomarkers in pre-clinical studies and the application of this information in clinical trials. Examples in the form of case studies have been provided that highlight the development, validation and use of these biomarkers in drug development. The challenges regarding the implementation of these biomarkers have also been discussed.
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Xu, X.H.N, Zu, Y. Electrochemiluminescence Detection in Bioanalysis in New Frontiers in Ultrasensitive Bioanalysis. ed. Zun X.H.N. (2007) John Wiley & Sons, Inc. 235-267.
Summary: The focus of this review paper is to discuss the fundamentals of electrochemiluminescence, the development of different ECL technologies, instrumentation, applications and the unique features of ECL method. Representative ECL assays and their applications in bioanalysis, and the current research in ECL mechanisms, new ECL emitters and coreactors have been presented.
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Simpson, P.B. (2006) A Model for Efficient Assay Development and Screening at a Small Research Site. J. Assoc. Lab Automation. Vol. 11(2):100-109.
Summary: This is a tutorial that describes a model in which a centralized assay team is developed which is highly skilled, has appropriate automation resources, is modest in expense and recruitment levels and has high productivity and efficiency. Such a group is capable of supporting all automated assay development and multiple types of screening.
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| Ramm, P. (2005) Image-based screening: a technology in transition. Curr Opin Biotechnol. Vol. 16(1):41-8.
fluorescence polarization (FP), fluorescence resonance energy transfer (FRET), SIBS, scintillation, ViewLux, LEADseeker
Summary: This review article describes the applications of image-based screening technology, including whole well biochemical screening, whole well cell screening, array screening, point target screening and subcellular screening. IBS, which is the analysis of data from digital images, is applied in screening for leads in drug discovery.
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Debad, J.D., Glezer, E.N., Wohlstadter, J.N., Sigal, G.B. (2004) Clinical and Biological Applications of ECL. in Electrogenerated Chemiluminescence, ed. A.J. Bard. Marcel Dekker, New York. 43-78.
Summary: This book chapter is a detailed review on the principle, instrumentation and assay formats of electrochemiluminescence technology, and the applications of these assays in the fields of life science research, clinical testing, food and water testing, and the detection of biowarfare agents.
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Pluskal, M.G., Bachmann, J. (2004) Advancing applications of microarrays. Drug Discov Today. Vol. 9(2):61-3.
Summary: This article lists the proceedings of Chips to Hits Conference, in which the developments in microarray technology, new surface chemistry and engineering methods as well as emerging technologies for target identification were discussed. Additionally, an overview of intellectual property and FDA regulation in product development was also provided.
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| Elands, J., Botwood, M.P. (2002) Meeting the challenges in screening. Drug Discov Today. Vol. 7(23):1162-4.
MESO SCALE DISCOVERY, Virtual Arrays, CellCards, zinc finger, Sangamo Biosciences, High Throughput Genomics
Summary: This review lists the proceedings of Society for Biomolecular Screening Eighth Annual Conference, held in Netherlands, in which novel screening methods, image based high content screening, different multiplexed immunoassay systems, use of computation for biological data analysis and structure function relationships of biological molecules were discussed.
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Bard, A.J., Debad, J.D., Leland, J.K., Sigal, G.B., Wilbur, J.L., Wohlstadter, J.N. (2000) Chemiluminescence, Electrogenerated. in Encyclopedia of Analytical Chemistry, ed. Meyers, R.A. John Wiley & Sons, Chichester. 9842-9849.
Summary: This is a review paper on the theory, principles, analytical applications and instrumentation of electrochemiluminescent assays.
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