Phosphoproteins and Intracellular Markers View Singleplex Assays View Multiplex Assays Singleplex Assays 4E-BP1 Phospho-4E-BP1 (Thr 37/46) Assay Total 4E-BP1 Assay Akt Phospho-Akt (Ser 473) Assay Phospho-Akt (Ser 473) Assay (384-well) Phospho-Akt (Thr 308) Assay Phospho-Akt (Thr 308) Assay (384-well) Total Akt Assay Aurora A Phospho-Aurora A (Thr 288) Assay Total Aurora A Assay BAD Phospho-BAD Assay Total BAD Assay Caspase-3 Cleaved Caspase-3 Assay Cleaved Caspase-3 Assay (384-well) EGFR Phospho-EGFR (Tyr 1173) Assay Phospho-EGFR (Tyr 1068) Assay Total EGFR Assay eIF2a Total eIF2a Assay eIF4E Phospho-eIF4E Assay Total eIF4E Assay ErbB2 Phospho-ErbB2 Assay Total ErbB2 Assay ERK Phospho-ERK1/2 (T/Y: 202/204; 185/187) Assay Phospho-ERK1/2 (T/Y: 202/204; 185/187) Assay (384-well) Total ERK1/2 (T/Y: 202/204; 185/187) Assay Total ERK1/2 (T/Y: 202/204; 185/187) Assay (384-well) Phospho-ERK5 (Thr 218/Tyr 220) Assay FOXO3a Phospho-FOXO3a (Thr 32) Assay FRS2 Phospho-FRS2 (Tyr 196) Assay Phospho-FRS2 (Tyr 436) Assay GSK-3a Phospho-GSK-3a Assay GSK-3b Phospho-GSK-3b Assay Phospho-GSK-3b Assay (384-well) Total GSK-3b Assay HIF-1a HIF-1a Assay HIF-1a Assay (384-well) Histone 3 Phospho-Histone H3 Assay HSP27 Phospho-HSP27 Assay Phospho-HSP27 Assay (384-well) Phospho-HSP27 (Ser 78) Assay Phospho-HSP27 (Ser 78) Assay (384-well) Phospho-HSP27 (Ser 82) Assay Phospho-HSP27 (Ser 82) Assay (384-well) Total HSP27 Assay HSP70 Total HSP70 Assay IGF-1R Phospho-IGF-1R Assay (384-well) IRS-1 Phospho-IRS-1 Assay JNK Phospho-JNK Assay Phospho-JNK Assay (384-well) Total JNK Assay Jun Phospho-c-Jun (Ser 63) Assay Phospho-c-Jun (Ser 63) Assay (384-well) Kit Phospho-c-Kit Assay Phospho-c-Kit Assay (384-well) Total c-Kit Assay MAPKAPK2 Phospho-MAPKAPK2 Assay MEK Phospho-MEK1/2 Assay Phospho-MEK1/2 Assay (384-well) Total MEK1/2 Assay Total MEK1/2 Assay (384-well) Total MEK2 Assay Met Phospho-Met Assay Phospho-Met Assay (384-well) Total Met Assay Total Met Assay (384-well) mTOR Phospho-mTOR (Ser 2448) Assay NFkB (p65) Phospho-NFkB (Ser 468) Assay Phospho-NFkB (Ser 536) Assay Phospho-NFkB (Ser 536) Assay (384-well) p38 Phospho-p38 Assay Phospho-p38 Assay (384-well) Total p38 Assay p53 Phospho-p53 Assay Phospho-p53 Assay (384-well) Total p53 Assay p62 (SQSTM1) Total p62 (SQSTM1) Assay p70S6K Phospho-p70S6K Assay Phospho-p70S6K (Thr 389) Assay Phospho-p70S6K (Thr 389) Assay (384-well) Total p70S6K Assay PARP Cleaved PARP Assay PDGFR-b Phospho-PDGFR-b Assay Phospho-PDGFR-b Assay (384-well) PERK Phospho-PERK (Thr 980) Assay Phospho-PERK (Thr 981) Assay Total PERK Assay Pleckstrin Phospho-Pleckstrin (Ser) Assay PRAS40 Phospho-PRAS40 (Thr 246) Assay Total PRAS40 Assay Rb Phospho-Rb (Ser 608) Assay Phospho-Rb (Ser 780) Assay Total Rb Assay S6RP Phospho-S6RP (Ser 235/ 236) Assay Phospho-S6RP (Ser 235/ 236) Assay (384-well) Phospho-S6RP (Ser 240/ 244) Assay Phospho-S6RP (Ser 240/ 244) Assay (384-well) Total S6RP Assay SRC3 Total SRC3 Assay Smad1 Phospho-Smad1 (Ser 463/ 465) Assay STAT3 Phospho-STAT3 Assay Phospho-STAT3 Assay (384-well) STAT5a,b Phospho-STAT5a,b (Tyr 694) Assay Total STAT5a,b Assay Tau Phospho-Tau (Thr 231) Assay Total Tau Assay VASP Phospho-VASP Assay Total VASP Assay VEGFR-2 Phospho-VEGFR-2 Assay Phospho-VEGFR-2 Assay (384-well) Multiplex Assays Phospho (Ser 473)/Total Akt Assay Phospho /Total BAD Assay Cleaved /Total Caspase-3 Assay Phospho /Total EGFR Assay Phospho /Total ErbB2 Assay Phospho /Total ERK Assay Phospho /Total GSK-3b Assay Phospho /Total HSP27 Assay Phospho (Ser 78)/Total HSP27 Assay Phospho (Ser 82)/Total HSP27 Assay Phospho /Total JNK Assay Phospho /Total c-Kit Assay Phospho /Total MEK 1/2 Assay Phospho /Total Met Assay Phospho (Ser 2448)/Total mTOR Assay Phospho /Total p38 Assay Phospho /Total p53 Assay Phospho /Total p70S6K Assay Phospho (Ser 608) /Total Rb Assay Phospho (Ser 780) /Total Rb Assay Phospho (Ser 240/244) /Total S6RP Assay Phospho (Tyr 694) /Total STAT5a,b Assay Phospho/Total Tau Assay Phospho /Total VASP Assay Multiplexed MAPK Assay: Phospho-p38 / Phospho-ERK1/2 / Phospho-JNK Multiplexed MAPK (Total Protein) Assay: p38 / ERK1/2 / JNK Multiplexed Phosphoprotein Assay: Phospho-EGFR / Phospho-ErbB2 / Phospho-IGF1R Akt Signaling Panel: Phospho-p70S6K / Phospho-GSK-3b / Phospho-Akt Akt Signaling Panel (Total Protein): Total p70S6K / Total GSK-3b / Total Akt Akt Signaling Panel II: Phospho-p70S6K / Phospho-S6RP / Phospho-GSK-3b / Phospho-Akt Multiplex Apoptosis Assay: Phospho-p53 / Total p53 / Cleaved Caspase-3 / Cleaved PARP ERK-STAT3 Phosphorylation Cascade: Phospho-ERK1/2, Phospho-MEK1/2, Phospho-STAT3 Insulin Signaling Panel (Phosphoprotein) Insulin Signaling Panel (Total Protein)