Human Chemokine 9-Plex Base Kit

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The Human Chemokine 9-Plex Base Kit provides basic components for the simultaneous quantitative determination of both natural and recombinant human Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, MIP-1β, and TARC. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.

Specifications
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		Eotaxin, Eotaxin-3, IL-8, IP-10, MCP-1, MCP-4, MDC, TARC
Species		Human
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Capture Antibody		Eotaxin: Mouse Monoclonal MIP-1β: Mouse Monoclonal Eotaxin-3: Mouse Monoclonal TARC: Mouse Monoclonal IP-10: Mouse Monoclonal IL-8: Mouse Monoclonal MCP-1: Mouse Monoclonal MDC: Mouse Monoclonal MCP-4: Mouse Monoclonal
Detection Antibody		Eotaxin: Mouse Monoclonal MIP-1β: Goat Polyclonal Eotaxin-3: Goat Polyclonal TARC: Goat Polyclonal IP-10: Goat Polyclonal IL-8: Goat Polyclonal MCP-1: Mouse Monoclonal MDC: Rabbit Polyclonal MCP-4: Goat Polyclonal
LLOD (Sensitivity)		Eotaxin: 23 pg/mL MIP-1β: 7.5 pg/mL Eotaxin-3: 6.1 pg/mL TARC: 15 pg/mL IP-10: 12 pg/mL IL-8: 0.6 pg/mL MCP-1: 8.0 pg/mL MDC: 334 pg/mL MCP-4: 8.4 pg/mL
Dynamic Range		Eotaxin: 23-40000 pg/mL MIP-1β: 7.5-40000 pg/mL Eotaxin-3: 6.1-10000 pg/mL TARC: 15-40000 pg/mL IP-10: 12-10000 pg/mL IL-8: 0.6-2500 pg/mL MCP-1: 8.0-10000 pg/mL MDC: 334-400000 pg/mL MCP-4: 8.4-10000 pg/mL
Sample Type		Serum, Plasma
Recombinant standards		Recombinant proteins expressed in E. coli.
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		2-8 °C

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
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Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
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Instrument Support
1-301-947-2057

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Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		Human 96 well Base
SDS		Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x)

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